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Western Blot analysis of A549 and Mouse eye tissue using ALDH3A1 Polyclonal Antibody at dilution of 1:750
Western Blot analysis of A549 and Mouse eye tissue using ALDH3A1 Polyclonal Antibody at dilution of 1:750
Western Blot analysis of A549 and Mouse eye tissue using ALDH3A1 Polyclonal Antibody at dilution of 1:750

ALDH3A1 Polyclonal Antibody

E-AB-14534
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    ALDH3A1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.4mg/ml
  • Scientific Description
    Aldehyde dehydrogenases oxidize various aldehydes to the corresponding acids. They are involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. The enzyme encoded by this gene forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to UV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. The gene is located within the Smith-Magenis syndrome region on chromosome 17. Multiple alternatively spliced variants, encoding the same protein, have been identified.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ALDH3A1 using anti-ALDH3A1 antibody (A01121-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse stomach tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH3A1 antigen affinity purified polyclonal antibody (Catalog # A01121-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH3A1 at approximately 55 kDa. The expected band size for ALDH3A1 is at 55 kDa.
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