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(LEFT)Western blot analysis of anti-ALDH3A1 Antibody (Center) in Y79 cell line lysates (35ug/lane). ALDH3A1 (arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of anti-ALDH3A1 Antibody (Center) in mouse muscle tissue lysates (35ug/lane). ALDH3A1 (arrow) was detected using the purified Pab.
(LEFT)Western blot analysis of anti-ALDH3A1 Antibody (Center) in Y79 cell line lysates (35ug/lane). ALDH3A1 (arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of anti-ALDH3A1 Antibody (Center) in mouse muscle tissue lysates (35ug/lane). ALDH3A1 (arrow) was detected using the purified Pab.
(LEFT)Western blot analysis of anti-ALDH3A1 Antibody (Center) in Y79 cell line lysates (35ug/lane). ALDH3A1 (arrow) was detected using the purified Pab. (RIGHT)Western blot analysis of anti-ALDH3A1 Antibody (Center) in mouse muscle tissue lysates (35ug/lane). ALDH3A1 (arrow) was detected using the purified Pab.

ALDH3A1 Rabbit Polyclonal Antibody

AP14687PU-N
OriGene
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetALDH3A1
Price on request
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    ALDH3A1 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID218
  • Target name
    ALDH3A1
  • Target description
    aldehyde dehydrogenase 3 family member A1
  • Target synonyms
    ALDH3, ALDHIII, aldehyde dehydrogenase, dimeric NADP-preferring, aldehyde dehydrogenase isozyme 3, aldehyde dehydrogenase type III, stomach aldehyde dehydrogenase
  • Host
    Rabbit
  • Protein IDP30838
  • Protein Name
    Aldehyde dehydrogenase, dimeric NADP-preferring
  • Scientific Description
    ALDH3A1 rabbit polyclonal antibody, Purified
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ALDH3A1 using anti-ALDH3A1 antibody (A01121-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse stomach tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH3A1 antigen affinity purified polyclonal antibody (Catalog # A01121-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH3A1 at approximately 55 kDa. The expected band size for ALDH3A1 is at 55 kDa.
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