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Western Blot analysis of Rat kidney tissue using ALDH6A1 Polyclonal Antibody at dilution of 1:450
Western Blot analysis of Rat kidney tissue using ALDH6A1 Polyclonal Antibody at dilution of 1:450
Western Blot analysis of Rat kidney tissue using ALDH6A1 Polyclonal Antibody at dilution of 1:450

ALDH6A1 Polyclonal Antibody

E-AB-10769
Elabscience
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetALDH6A1
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Overview

  • Supplier
    Elabscience
  • Product Name
    ALDH6A1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.4 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4329
  • Target name
    ALDH6A1
  • Target description
    aldehyde dehydrogenase 6 family member A1
  • Target synonyms
    MMSADHA, MMSDH, methylmalonate-semialdehyde/malonate-semialdehyde dehydrogenase [acylating], mitochondrial, malonate-semialdehyde dehydrogenase (acetylating), methylmalonate-semialdehyde dehydrogenase [acylating], mitochondrial, mitochondrial acylating methylmalonate-semialdehyde dehydrogenase, testicular tissue protein Li 122
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ02252
  • Protein Name
    Methylmalonate-semialdehyde/malonate-semialdehyde dehydrogenase [acylating], mitochondrial
  • Scientific Description
    This protein belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. The product of this gene, a mitochondrial methylmalonate semialdehyde dehydrogenase, catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of ALDH6A1 using anti-ALDH6A1 antibody (A08868-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: rat kidney tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse NIH/3T3 whole cell lysates, Lane 7: human MCF-7 whole cell lysates, Lane 8: human HEPG2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDH6A1 antigen affinity purified polyclonal antibody (Catalog # A08868-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDH6A1 at approximately 58KD. The expected band size for ALDH6A1 is at 58KD.
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