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ALDOA antibody

70R-15675
Biosynth
Product group Antibodies
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More than 130 suppliers
ISO certified
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Overview

  • Supplier
    Biosynth
  • Product Name
    ALDOA antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 3. IHC analysis of ALDOA using anti-ALDOA antibody (A05022-3). ALDOA was detected in paraffin-embedded section of human Lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1microg/ml rabbit anti-ALDOA Antibody (A05022-3) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Aldolase Polyclonal Antibody, Unconjugated (bs-2406R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
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