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Western Blot analysis of HuvEc cells using Aldolase B Polyclonal Antibody
Western Blot analysis of HuvEc cells using Aldolase B Polyclonal Antibody
Western Blot analysis of HuvEc cells using Aldolase B Polyclonal Antibody

ALDOB Antibody

CSB-PA000863
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetALDOB
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Overview

  • Supplier
    Cusabio
  • Product Name
    ALDOB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID229
  • Target name
    ALDOB
  • Target description
    aldolase, fructose-bisphosphate B
  • Target synonyms
    ALDB; ALDO2; aldolase 2; aldolase B, fructose-bisphosphatase; aldolase B, fructose-bisphosphate; fructose-bisphosphate aldolase B; liver-type aldolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05062
  • Protein Name
    Fructose-bisphosphate aldolase B
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ALDOB using anti-ALDOB antibody (A03893-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: monkey liver tissue lysates, Lane 9: monkey kidney tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDOB antigen affinity purified polyclonal antibody (Catalog # A03893-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDOB at approximately 39KD. The expected band size for ALDOB is at 39KD.
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