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Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.
Immunohistochemical staining of human kidney shows strong cytoplasmic and nuclear positivity in cells in tubules.

Anti-ALDOB Antibody

HPA073201
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetALDOB
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ALDOB
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID229
  • Target name
    ALDOB
  • Target description
    aldolase, fructose-bisphosphate B
  • Target synonyms
    ALDB, ALDO2, fructose-bisphosphate aldolase B, aldolase 2, aldolase B, fructose-bisphosphatase, aldolase B, fructose-bisphosphate, liver-type aldolase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05062
  • Protein Name
    Fructose-bisphosphate aldolase B
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ALDOB using anti-ALDOB antibody (A03893-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: rat liver tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: monkey liver tissue lysates, Lane 9: monkey kidney tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALDOB antigen affinity purified polyclonal antibody (Catalog # A03893-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALDOB at approximately 39KD. The expected band size for ALDOB is at 39KD.
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