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ICC/IF analysis of U87-MG cells using GTX57650 Aldolase C antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
ICC/IF analysis of U87-MG cells using GTX57650 Aldolase C antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
ICC/IF analysis of U87-MG cells using GTX57650 Aldolase C antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100

Aldolase C antibody [AT2E11]

GTX57650
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetALDOC
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Overview

  • Supplier
    GeneTex
  • Product Name
    Aldolase C antibody [AT2E11]
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT2E11
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID230
  • Target name
    ALDOC
  • Target description
    aldolase, fructose-bisphosphate C
  • Target synonyms
    ALDC; aldolase 3; aldolase C, fructose-bisphosphate; brain-type aldolase; epididymis secretory sperm binding protein; fructoaldolase C; fructose-1,6-biphosphate triosephosphate lyase; fructose-bisphosphate aldolase C
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP09972
  • Protein Name
    Fructose-bisphosphate aldolase C
  • Scientific Description
    This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Aldolase C/ALDOC using anti-Aldolase C/ALDOC antibody (A05296-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aldolase C/ALDOC antigen affinity purified polyclonal antibody (Catalog # A05296-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aldolase C/ALDOC at approximately 39 kDa. The expected band size for Aldolase C/ALDOC is at 39 kDa.
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