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Aldolase C antibody [N1C1]

GTX102284
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
DownloadDatasheet
Product group Antibodies
TargetALDOC
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Overview

  • Supplier
    GeneTex
  • Product Name
    Aldolase C antibody [N1C1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-Fr: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.28 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID230
  • Target name
    ALDOC
  • Target description
    aldolase, fructose-bisphosphate C
  • Target synonyms
    ALDC; aldolase 3; aldolase C, fructose-bisphosphate; brain-type aldolase; epididymis secretory sperm binding protein; fructoaldolase C; fructose-1,6-biphosphate triosephosphate lyase; fructose-bisphosphate aldolase C
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09972
  • Protein Name
    Fructose-bisphosphate aldolase C
  • Scientific Description
    This gene encodes a member of the class I fructose-biphosphate aldolase gene family. Expressed specifically in the hippocampus and Purkinje cells of the brain, the encoded protein is a glycolytic enzyme that catalyzes the reversible aldol cleavage of fructose-1,6-biphosphate and fructose 1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde-3-phosphate or glyceraldehyde, respectively. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • MEOX2 Regulates the Growth and Survival of Glioblastoma Stem Cells by Modulating Genes of the Glycolytic Pathway and Response to Hypoxia. Proserpio C et al., 2022 May 6, Cancers (Basel)
    Read more
  • Traumatically injured astrocytes release a proteomic signature modulated by STAT3-dependent cell survival. Levine J et al., 2016 May, Glia
    Read more
  • Integrating transcriptomics and proteomics to show that tanshinone IIA suppresses cell growth by blocking glucose metabolism in gastric cancer cells. Lin LL et al., 2015 Feb 5, BMC Genomics
    Read more
  • Quantitative proteomic analysis of human lung tumor xenografts treated with the ectopic ATP synthase inhibitor citreoviridin. Wu YH et al., 2013, PLoS One
    Read more

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Figure 1. Western blot analysis of Aldolase C/ALDOC using anti-Aldolase C/ALDOC antibody (A05296-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: rat C6 whole cell lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Aldolase C/ALDOC antigen affinity purified polyclonal antibody (Catalog # A05296-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Aldolase C/ALDOC at approximately 39 kDa. The expected band size for Aldolase C/ALDOC is at 39 kDa.
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