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ALKBH5 Antibody

LS-C747075
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetALKBH5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ALKBH5 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 43kDa/44kDa/52kDa, while the observed MW by Western blot was 51kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:500 - 1:2000) The predicted MW is 43kDa/44kDa/52kDa, while the observed MW by Western blot was 51kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54890
  • Target name
    ALKBH5
  • Target description
    alkB homolog 5, RNA demethylase
  • Target synonyms
    ABH5; AlkB family member 5, RNA demethylase; alkB, alkylation repair homolog 5; alkylated DNA repair protein alkB homolog 5; alpha-ketoglutarate-dependent dioxygenase alkB homolog 5; OFOXD; OFOXD1; oxoglutarate and iron-dependent oxygenase domain containing; probable alpha-ketoglutarate-dependent dioxygenase ABH5; RNA demethylase ALKBH5
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ALKBH5 using anti-ALKBH5 antibody (A03360). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human U87 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALKBH5 antigen affinity purified polyclonal antibody (Catalog # A03360) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALKBH5 at approximately 52 kDa. The expected band size for ALKBH5 is at 44 kDa.
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