![Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (GTX637965) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (GTX637965) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637965/GTX637965_T-44886_20230224_WB_R_testis_23030219_426.webp)
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (GTX637965) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
ALKBH5 antibody [HL2061]
GTX637965
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetALKBH5
Overview
- SupplierGeneTex
- Product NameALKBH5 antibody [HL2061]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL2061
- Concentration2 mg/ml
- ConjugateUnconjugated
- Gene ID54890
- Target nameALKBH5
- Target descriptionalkB homolog 5, RNA demethylase
- Target synonymsABH5, OFOXD, OFOXD1, RNA demethylase ALKBH5, AlkB family member 5, RNA demethylase, alkB, alkylation repair homolog 5, alkylated DNA repair protein alkB homolog 5, alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, oxoglutarate and iron-dependent oxygenase domain containing, probable alpha-ketoglutarate-dependent dioxygenase ABH5
- HostRabbit
- IsotypeIgG
- Protein IDQ6P6C2
- Protein NameRNA demethylase ALKBH5
- Scientific DescriptionEnables mRNA N6-methyladenosine dioxygenase activity. Involved in RNA metabolic process; mRNA export from nucleus; and response to hypoxia. Located in Golgi apparatus; cytosol; and nuclear speck. [provided by Alliance of Genome Resources, Apr 2022]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
![Wild-type (WT) and ALKBH5 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (GTX637965) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Wild-type (WT) and ALKBH5 knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (GTX637965) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637965/GTX637965_T-44886_20230224_WB_KO_watermark_23030219_166.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (GTX637965) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (GTX637965) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637965/GTX637965_T-44886_20230303_WB_R_23030717_748.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (GTX637965) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (GTX637965) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637965/GTX637965_T-44886_20230303_WB_M_23030717_696.webp)
![Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (GTX637965) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALKBH5 antibody [HL2061] (GTX637965) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637965/GTX637965_45026_20230428_WB_23050223_773.webp)





