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alpha 1-adaptin Antibody (YA2295, Rabbit)

HY-P82550
MedChem Express
TargetAP2A1
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    alpha 1-adaptin Antibody (YA2295, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID160
  • Target name
    AP2A1
  • Target description
    adaptor related protein complex 2 subunit alpha 1
  • Target synonyms
    100 kDa coated vesicle protein A; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptin, alpha A; adaptor protein complex AP-2 subunit alpha-1; adaptor related protein complex 2 alpha 1 subunit; ADTAA; alpha1-adaptin; alpha-adaptin A; AP-2 complex subunit alpha-1; AP2-ALPHA; CLAPA1; clathrin assembly protein complex 2 alpha-A large chain; clathrin-associated/assembly/adaptor protein, large, alpha 1; plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    alpha 1-adaptin Antibody (YA2295) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA2295), targeting alpha 1-adaptin, with a predicted molecular weight of 108 kDa (observed band size: 108 kDa). alpha 1-adaptin Antibody (YA2295) can be used for WB, IHC-P, ICC/IF, FC experiment in human, mouse, rat background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Alpha Adaptin/AP2A1 using anti-Alpha Adaptin/AP2A1 antibody (A08806-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Adaptin/AP2A1 antigen affinity purified polyclonal antibody (Catalog # A08806-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Adaptin/AP2A1 at approximately 108 kDa. The expected band size for Alpha Adaptin/AP2A1 is at 108 kDa.
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