Bio-Connect

AP2A1 antibody

GTX132948
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetAP2A1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    AP2A1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.33 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID160
  • Target name
    AP2A1
  • Target description
    adaptor related protein complex 2 subunit alpha 1
  • Target synonyms
    100 kDa coated vesicle protein A; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptin, alpha A; adaptor protein complex AP-2 subunit alpha-1; adaptor related protein complex 2 alpha 1 subunit; ADTAA; alpha1-adaptin; alpha-adaptin A; AP-2 complex subunit alpha-1; AP2-ALPHA; CLAPA1; clathrin assembly protein complex 2 alpha-A large chain; clathrin-associated/assembly/adaptor protein, large, alpha 1; plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO95782
  • Protein Name
    AP-2 complex subunit alpha-1
  • Scientific Description
    This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Everest Biotech
ApplicationsWestern Blot, ELISA
TargetAP2A1
  • SizePrice
Product group Antibodies
Bethyl Laboratories
ApplicationsImmunoPrecipitation, Western Blot
TargetAP2A1
  • SizePrice
Product group Antibodies
Assay Genie
TargetAP2A1
  • SizePrice
Product group Antibodies
Bioss Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetAP2A1
  • SizePrice
Figure 1. Western blot analysis of Alpha Adaptin/AP2A1 using anti-Alpha Adaptin/AP2A1 antibody (A08806-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Adaptin/AP2A1 antigen affinity purified polyclonal antibody (Catalog # A08806-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Adaptin/AP2A1 at approximately 108 kDa. The expected band size for Alpha Adaptin/AP2A1 is at 108 kDa.
Product group Antibodies
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetAP2A1
  • SizePrice
Product group Antibodies
Antibodies.com
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetAP2A1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetAP2A1
  • SizePrice
ICC/IF analysis of HeLa cells using GTX22730 alpha Adaptin antibody [AP6]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature
Product group Antibodies
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
TargetAP2A1
  • SizePrice