
Western Blot of Goat anti Alpha-1anti-Trypsin antibody. Lane 1: Alpha-1anti-Trypsin under reducing conditions. Lane 2: none. Load: 1 microg per lane. Primary antibody: Alpha-1anti-Trypsin antibody (GTX27633) at 1:3000 for overnight at 4°C. Secondary antibody: Flourescent dye 488 conjugated Donkey anti goat secondary antibody at 1:10,000 for 45 min at RT. Block: overnight at 4°C. Predicted/Observed size: 55kDa for a-1 Anti-Trypsin. Other band(s): none
alpha 1 Antitrypsin antibody
GTX27633
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetSERPINA1
Overview
- SupplierGeneTex
- Product Namealpha 1 Antitrypsin antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000-1:10000. ELISA: 1:20000-1:100000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration10 mg/ml
- ConjugateUnconjugated
- Gene ID5265
- Target nameSERPINA1
- Target descriptionserpin family A member 1
- Target synonymsA1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, nNIF, alpha-1-antitrypsin, alpha-1 antitrypsin, alpha-1 protease inhibitor, alpha-1 proteinase inhibitor, alpha-1-antiproteinase, anti-elastase, epididymis secretory sperm binding protein, protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin, serine (or cysteine) proteinase inhibitor, clade A, member 1, serpin A1, serpin peptidase inhibitor clade A (alpha-1antiproteinase, antitrypsin) member 1, serpin peptidase inhibitor clade A member 1, serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
- HostGoat
- IsotypeIgG
- Protein IDP01009
- Protein NameAlpha-1-antitrypsin
- Scientific Descriptionalpha 1 Antitrypsin is a plasma protein synthesised in the liver. It consists of a single polypeptide chain and has a molecular weight of 52 kDa. A serine protease inhibitor, its primary function is the protection of lung tissue from the action of neutrophil elastase. The average level of alpha 1 Antitrypsin in plasma is 1.3 g/l.
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203




![FACS analysis of 70% ethanol fixed HepG2 cells using GTX15777 alpha 1 Antitrypsin antibody [TMF1#4B5]. Red histogram : Primary antibody Pink histogram : mouse isotype control Fixation : 70% ethanol for 10 minutes Permeabilization : 0.25% Trito X-100 for 20 minutes Dilution : 3-5 microg/million cells in 2.5% BSA for 30 minutes at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX15777/GTX15777_116_FACS_w_23060620_281.webp)





![WB analysis of human plasma (1) and NIH3T3 cell lysate (2) using GTX83379 alpha 1 Antitrypsin antibody [2B12].](https://www.genetex.com/upload/website/prouct_img/normal/GTX83379/GTX83379_20170912_WB_w_23061322_987.webp)
![IHC-P analysis of human kidney tissue using GTX83663 alpha 1 Antitrypsin antibody [11G2]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX83663/GTX83663_1677_IHC-P_w_23061420_318.webp)