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ProductsBack/Anti-SERPINA1 [SERPINA1-1]

Anti-SERPINA1 [SERPINA1-1]

AB03820-10.0
Absolute Antibody
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking
Product group Antibodies
TargetSERPINA1
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-SERPINA1 [SERPINA1-1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    This antibody was used for indirect ELISA, Western blot (WB), and immunohistochemistry (IHC) in internal testing. Also, this antibody is known to inhibit the enzyme SERPINA1.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SERPINA1-1
  • Gene ID5265
  • Target name
    SERPINA1
  • Target description
    serpin family A member 1
  • Target synonyms
    A1A; A1AT; AAT; alpha-1 antitrypsin; alpha-1 protease inhibitor; alpha-1 proteinase inhibitor; alpha-1-antiproteinase; alpha-1-antitrypsin; alpha-1-antitrypsin short transcript variant 1C4; alpha-1-antitrypsin short transcript variant 1C5; alpha1AT; anti-elastase; epididymis secretory sperm binding protein; nNIF; PI; PI1; PRO2275; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; serine (or cysteine) proteinase inhibitor, clade A, member 1; serpin A1; serpin peptidase inhibitor clade A (alpha-1antiproteinase, antitrypsin) member 1; serpin peptidase inhibitor clade A member 1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP01009
  • Protein Name
    Alpha-1-antitrypsin
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SERPINA1 using anti-SERPINA1 antibody (PB10097). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: HELA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA1 antigen affinity purified polyclonal antibody (Catalog # PB10097) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINA1 at approximately 52 kDa. The expected band size for SERPINA1 is at 47 kDa.
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