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ProductsBack/alpha 1 Fetoprotein (AFP) Mouse Monoclonal Antibody [Clone ID: 131-12210 (TB-4B2-10)]

alpha 1 Fetoprotein (AFP) Mouse Monoclonal Antibody [Clone ID: 131-12210 (TB-4B2-10)]

AM00787PU-N
OriGene
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetAFP
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1 mg
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Overview

  • Supplier
    OriGene
  • Product Name
    alpha 1 Fetoprotein (AFP) Mouse Monoclonal Antibody [Clone ID: 131-12210 (TB-4B2-10)]
  • Delivery Days Customer
    14
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    131-12210 (TB-4B2-10)
  • Gene ID174
  • Target name
    AFP
  • Target description
    alpha fetoprotein
  • Target synonyms
    AFPD, FETA, HPAFP, alpha-fetoprotein, alpha-1-fetoprotein, alpha-fetoglobulin
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP02771
  • Protein Name
    Alpha-fetoprotein
  • Scientific Description
    alpha 1 Fetoprotein (AFP) mouse monoclonal antibody, clone 131-12210 (TB-4B2-10), Purified
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin-embedded Rat Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with AFP (A2) Monoclonal Antibody, Unconjugated (bsm-1621M) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody (bs-0295G-cy3) for 90 minutes, and DAPI for nuclei staining.
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Figure 1. Western blot analysis of alpha 1 Fetoprotein/AFP using anti-alpha 1 Fetoprotein/AFP antibody (A00522-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-alpha 1 Fetoprotein/AFP antigen affinity purified polyclonal antibody (Catalog # A00522-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for alpha 1 Fetoprotein/AFP at approximately 69 kDa. The expected band size for alpha 1 Fetoprotein/AFP is at 69 kDa.
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