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alpha 2a Adrenergic Receptor antibody [HL3723]

GTX641913
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetADRA2A
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Overview

  • Supplier
    GeneTex
  • Product Name
    alpha 2a Adrenergic Receptor antibody [HL3723]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL3723
  • Conjugate
    Unconjugated
  • Gene ID150
  • Target name
    ADRA2A
  • Target description
    adrenoceptor alpha 2A
  • Target synonyms
    ADRA2; ADRA2R; ADRAR; adrenergic, alpha-2A-, receptor; alpha-2 adrenergic receptor subtype C10; alpha-2A adrenergic receptor; alpha-2A adrenoceptor; alpha-2A adrenoreceptor; ALPHA2AAR; alpha-2AAR subtype C10; alpha-2-adrenergic receptor, platelet type; ZNF32
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP08913
  • Protein Name
    Alpha-2A adrenergic receptor
  • Scientific Description
    Alpha-2-adrenergic receptors are members of the G protein-coupled receptor superfamily. They include 3 highly homologous subtypes: alpha2A, alpha2B, and alpha2C. These receptors have a critical role in regulating neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. Studies in mouse revealed that both the alpha2A and alpha2C subtypes were required for normal presynaptic control of transmitter release from sympathetic nerves in the heart and from central noradrenergic neurons; the alpha2A subtype inhibited transmitter release at high stimulation frequencies, whereas the alpha2C subtype modulated neurotransmission at lower levels of nerve activity. This gene encodes alpha2A subtype and it contains no introns in either its coding or untranslated sequences. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of alpha 2a Adrenergic Receptor/ADRA2A using anti-alpha 2a Adrenergic Receptor/ADRA2A antibody (A00883-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates, Lane 2: rat small intestine tissue lysates, Lane 3: mouse pancreas tissue lysates, Lane 4: mouse small intestine tissue lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-alpha 2a Adrenergic Receptor/ADRA2A antigen affinity purified polyclonal antibody (Catalog # A00883-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for alpha 2a Adrenergic Receptor/ADRA2A at approximately 55 kDa. The expected band size for alpha 2a Adrenergic Receptor/ADRA2A is at 49 kDa.
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