ICC/IF analysis of HeLa cells transiently transfected with alpha Actinin plasmid using GTX84959 alpha Actinin antibody [7A4].
![ICC/IF analysis of HeLa cells using GTX84959 alpha Actinin antibody [7A4].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84959/GTX84959_1299_ICCIF_w_23061420_391.webp "ICC/IF analysis of HeLa cells using GTX84959 alpha Actinin antibody [7A4].")
![IHC-P analysis of human bladder tissue using GTX84959 alpha Actinin antibody [7A4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84959/GTX84959_3288_IHC-P_w_23061420_346.webp "IHC-P analysis of human bladder tissue using GTX84959 alpha Actinin antibody [7A4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human breast tissue using GTX84959 alpha Actinin antibody [7A4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84959/GTX84959_3289_IHC-P_w_23061420_565.webp "IHC-P analysis of human breast tissue using GTX84959 alpha Actinin antibody [7A4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human kidney tissue using GTX84959 alpha Actinin antibody [7A4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84959/GTX84959_3290_IHC-P_w_23061420_789.webp "IHC-P analysis of human kidney tissue using GTX84959 alpha Actinin antibody [7A4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human liver tissue using GTX84959 alpha Actinin antibody [7A4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84959/GTX84959_3291_IHC-P_w_23061420_376.webp "IHC-P analysis of human liver tissue using GTX84959 alpha Actinin antibody [7A4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human ovary tissue using GTX84959 alpha Actinin antibody [7A4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84959/GTX84959_3292_IHC-P_w_23061420_210.webp "IHC-P analysis of human ovary tissue using GTX84959 alpha Actinin antibody [7A4]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![WB analysis of various samples using GTX84959 alpha Actinin antibody [7A4]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84959/GTX84959_3590_WB_w_23061420_176.webp "WB analysis of various samples using GTX84959 alpha Actinin antibody [7A4]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of various cell lines using GTX84959 alpha Actinin antibody [7A4]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84959/GTX84959_3591_WB_w_23061420_135.webp "WB analysis of various cell lines using GTX84959 alpha Actinin antibody [7A4]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of wild type and alpha Actinin knock out HeLa cell lysate(10microg per lane) using GTX84959 alpha Actinin antibody [7A4]. Dilution : 1:500](https://www.genetex.com/upload/website/prouct_img/normal/GTX84959/GTX84959_4744_20181228_WB_w_23061420_340.webp "WB analysis of wild type and alpha Actinin knock out HeLa cell lysate(10microg per lane) using GTX84959 alpha Actinin antibody [7A4]. Dilution : 1:500")
ICC/IF analysis of HeLa cells transiently transfected with alpha Actinin plasmid using GTX84959 alpha Actinin antibody [7A4].
alpha Actinin antibody [7A4]
GTX84959
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetACTN1
Overview
- SupplierGeneTex
- Product Namealpha Actinin antibody [7A4] - KO/KD, Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:2000. ICC/IF: 1:100. IHC-P: 1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID7A4
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID87
- Target nameACTN1
- Target descriptionactinin alpha 1
- Target synonymsactinin 1 smooth muscle; alpha-actinin-1; BDPLT15; epididymis secretory sperm binding protein; F-actin cross-linking protein; non-muscle alpha-actinin-1
- HostMouse
- IsotypeIgG2a
- Protein IDP12814
- Protein NameAlpha-actinin-1
- Scientific DescriptionF-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
- ReactivityHuman, Monkey, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- A novel and potent brain penetrant inhibitor of extracellular vesicle release. Rojas C et al., 2019 Oct, Br J PharmacolRead more
- Sensory contact to the stressor prevents recovery from structural and functional heart damage following psychosocial trauma. Foertsch S et al., 2019 Aug, Brain Behav ImmunRead more
- Functional and biochemical responses of skeletal muscle following a moderate degree of systemic iron loading in mice. Liang C et al., 2019 Apr 1, J Appl Physiol (1985)Read more