![ICC/IF analysis of HeLa cells using GTX22730 alpha Adaptin antibody [AP6]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature ICC/IF analysis of HeLa cells using GTX22730 alpha Adaptin antibody [AP6]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22730/GTX22730_394_ICC-IF_w_23060620_674.webp)
ICC/IF analysis of HeLa cells using GTX22730 alpha Adaptin antibody [AP6]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature
alpha Adaptin antibody [AP6]
GTX22730
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityBovine, Hamster, Human, Mouse, Primate, Rat
TargetAP2A1
Overview
- SupplierGeneTex
- Product Namealpha Adaptin antibody [AP6]
- Delivery Days Customer9
- Antibody SpecificityThis antibody recognizes the products of both alpha-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha adaptin found in neurons.
- Application Supplier NoteWB: 1:200. ICC/IF: 1-2 microg/ml. FACS: 1microg per 106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDAP6
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID160
- Target nameAP2A1
- Target descriptionadaptor related protein complex 2 subunit alpha 1
- Target synonyms100 kDa coated vesicle protein A; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptin, alpha A; adaptor protein complex AP-2 subunit alpha-1; adaptor related protein complex 2 alpha 1 subunit; ADTAA; alpha1-adaptin; alpha-adaptin A; AP-2 complex subunit alpha-1; AP2-ALPHA; CLAPA1; clathrin assembly protein complex 2 alpha-A large chain; clathrin-associated/assembly/adaptor protein, large, alpha 1; plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
- HostMouse
- IsotypeIgG1
- Protein IDO95782
- Protein NameAP-2 complex subunit alpha-1
- Scientific DescriptionThis gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]
- ReactivityBovine, Hamster, Human, Mouse, Primate, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203