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ICC/IF analysis of HeLa cells using GTX22730 alpha Adaptin antibody [AP6]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature
ICC/IF analysis of HeLa cells using GTX22730 alpha Adaptin antibody [AP6]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature
ICC/IF analysis of HeLa cells using GTX22730 alpha Adaptin antibody [AP6]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature

alpha Adaptin antibody [AP6]

GTX22730
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
Product group Antibodies
ReactivityBovine, Hamster, Human, Mouse, Primate, Rat
TargetAP2A1
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Overview

  • Supplier
    GeneTex
  • Product Name
    alpha Adaptin antibody [AP6]
  • Delivery Days Customer
    9
  • Antibody Specificity
    This antibody recognizes the products of both alpha-adaptin genes, alpha A and alpha C as well as an alternatively spliced isoform of alpha adaptin found in neurons.
  • Application Supplier Note
    WB: 1:200. ICC/IF: 1-2 microg/ml. FACS: 1microg per 106 cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin, Neutralisation/Blocking
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AP6
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID160
  • Target name
    AP2A1
  • Target description
    adaptor related protein complex 2 subunit alpha 1
  • Target synonyms
    100 kDa coated vesicle protein A; adapter-related protein complex 2 alpha-1 subunit; adapter-related protein complex 2 subunit alpha-1; adaptin, alpha A; adaptor protein complex AP-2 subunit alpha-1; adaptor related protein complex 2 alpha 1 subunit; ADTAA; alpha1-adaptin; alpha-adaptin A; AP-2 complex subunit alpha-1; AP2-ALPHA; CLAPA1; clathrin assembly protein complex 2 alpha-A large chain; clathrin-associated/assembly/adaptor protein, large, alpha 1; plasma membrane adaptor HA2/AP2 adaptin alpha A subunit
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO95782
  • Protein Name
    AP-2 complex subunit alpha-1
  • Scientific Description
    This gene encodes the alpha 1 adaptin subunit of the adaptor protein 2 (AP-2) complex found in clathrin coated vesicles. The AP-2 complex is a heterotetramer consisting of two large adaptins (alpha or beta), a medium adaptin (mu), and a small adaptin (sigma). The complex is part of the protein coat on the cytoplasmic face of coated vesicles which links clathrin to receptors in vesicles. Alternative splicing of this gene results in two transcript variants encoding two different isoforms. A third transcript variant has been described, but its full length nature has not been determined. [provided by RefSeq, Jul 2008]
  • Reactivity
    Bovine, Hamster, Human, Mouse, Primate, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203