ICC/IF analysis of COS7 cells transiently transfected with alpha B Crystallin plasmid using GTX84657 alpha B Crystallin antibody [6D11].
![ICC/IF analysis of HT29 cells using GTX84657 alpha B Crystallin antibody [6D11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84657/GTX84657_1176_ICCIF_w_23061420_887.webp "ICC/IF analysis of HT29 cells using GTX84657 alpha B Crystallin antibody [6D11].")
![IHC-P analysis of human thyroid carcinoma tissue using GTX84657 alpha B Crystallin antibody [6D11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.](https://www.genetex.com/upload/website/prouct_img/normal/GTX84657/GTX84657_2992_IHC-P_w_23061420_614.webp "IHC-P analysis of human thyroid carcinoma tissue using GTX84657 alpha B Crystallin antibody [6D11]. Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100oC for 10min.")
![IHC-P analysis of human smooth muscle cancer tissue using GTX84657 alpha B Crystallin antibody [6D11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84657/GTX84657_2993_IHC-P_w_23061420_841.webp "IHC-P analysis of human smooth muscle cancer tissue using GTX84657 alpha B Crystallin antibody [6D11].")
![IP analysis of DDDDK tagged alpha B Crystallin overexpressed HEK293T lysate using GTX84657 alpha B Crystallin antibody [6D11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate](https://www.genetex.com/upload/website/prouct_img/normal/GTX84657/GTX84657_3331_IP_w_23061420_628.webp "IP analysis of DDDDK tagged alpha B Crystallin overexpressed HEK293T lysate using GTX84657 alpha B Crystallin antibody [6D11].After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDDDK polyclonal antibody. IP reaction : 2microg antibody / 500microl cell lysate")
![WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84657 alpha B Crystallin antibody [6D11]. Loading : 10 ug per lane Dilution : 1:200](https://www.genetex.com/upload/website/prouct_img/normal/GTX84657/GTX84657_4139_WB_w_23061420_236.webp "WB analysis of human tissues (Lane 1-Testis ; Lane 2-Omentum ; Lane 3-Uterus ; Lane 4-Breast ; Lane 5-Brain ; Lane 6-Liver ; Lane 7-Ovary ; Lane 8-Thyroid gland ; Lane 9-colon ; Lane 10-spleen) using GTX84657 alpha B Crystallin antibody [6D11]. Loading : 10 ug per lane Dilution : 1:200")
![WB analysis of HEK293T cells transfected with alpha B Crystallin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84657 alpha B Crystallin antibody [6D11]. Loading : 5 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84657/GTX84657_4534_WB_w_23061420_608.webp "WB analysis of HEK293T cells transfected with alpha B Crystallin plasmid (Right) or empty vector (Left) for 48 hrs using GTX84657 alpha B Crystallin antibody [6D11]. Loading : 5 ug per lane")
![WB analysis of various cell lines using GTX84657 alpha B Crystallin antibody [6D11]. Loading : 35 ug per lane](https://www.genetex.com/upload/website/prouct_img/normal/GTX84657/GTX84657_4536_WB_w_23061420_651.webp "WB analysis of various cell lines using GTX84657 alpha B Crystallin antibody [6D11]. Loading : 35 ug per lane")
![FACS analysis of HEK293T cells transfected with either alpha B Crystallin plasmid(Red) or empty vector control plasmid(Blue) using GTX84657 alpha B Crystallin antibody [6D11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX84657/GTX84657_493_FACS_w_23061420_791.webp "FACS analysis of HEK293T cells transfected with either alpha B Crystallin plasmid(Red) or empty vector control plasmid(Blue) using GTX84657 alpha B Crystallin antibody [6D11].")
ICC/IF analysis of COS7 cells transiently transfected with alpha B Crystallin plasmid using GTX84657 alpha B Crystallin antibody [6D11].
alpha B Crystallin antibody [6D11]
GTX84657
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Rat
TargetCRYAB
Overview
- SupplierGeneTex
- Product Namealpha B Crystallin antibody [6D11] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500. ICC/IF: 1:50-1:100. IHC-P: 1:50. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID6D11
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1410
- Target nameCRYAB
- Target descriptioncrystallin alpha B
- Target synonymsalpha-crystallin B chain; CMD1II; CRYA2; CTPP2; CTRCT16; epididymis secretory protein Li 101; heat shock protein beta-5; heat-shock 20 kD like-protein; HEL-S-101; HSPB5; MFM2; renal carcinoma antigen NY-REN-27; rosenthal fiber component
- HostMouse
- IsotypeIgG1
- Protein IDP02511
- Protein NameAlpha-crystallin B chain
- Scientific DescriptionMay contribute to the transparency and refractive index of the lens.
- ReactivityHuman, Monkey, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203