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ProductsBack/alpha B Crystallin antibody [6D11]
ICC/IF analysis of COS7 cells transiently transfected with alpha B Crystallin plasmid using GTX84657 alpha B Crystallin antibody [6D11].
ICC/IF analysis of COS7 cells transiently transfected with alpha B Crystallin plasmid using GTX84657 alpha B Crystallin antibody [6D11].
ICC/IF analysis of COS7 cells transiently transfected with alpha B Crystallin plasmid using GTX84657 alpha B Crystallin antibody [6D11].

alpha B Crystallin antibody [6D11]

GTX84657
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCRYAB
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Overview

  • Supplier
    GeneTex
  • Product Name
    alpha B Crystallin antibody [6D11] - Overexpression Validated
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500. ICC/IF: 1:50-1:100. IHC-P: 1:50. FACS: 1:100. IP: 2ug/500ul. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6D11
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID1410
  • Target name
    CRYAB
  • Target description
    crystallin alpha B
  • Target synonyms
    alpha-crystallin B chain; CMD1II; CRYA2; CTPP2; CTRCT16; epididymis secretory protein Li 101; heat shock protein beta-5; heat-shock 20 kD like-protein; HEL-S-101; HSPB5; MFM2; renal carcinoma antigen NY-REN-27; rosenthal fiber component
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP02511
  • Protein Name
    Alpha-crystallin B chain
  • Scientific Description
    May contribute to the transparency and refractive index of the lens.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Alpha B Crystallin/CRYAB using anti-Alpha B Crystallin/CRYAB antibody (A03496-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat eye tissue lysates, Lane 2: mouse eye tissue lysates, Lane 3: rat heart tissue lysates, Lane 4: mouse heart tissue lysates, Lane 5: monkey heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha B Crystallin/CRYAB antigen affinity purified polyclonal antibody (Catalog # A03496-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha B Crystallin/CRYAB2 at approximately 20KD. The expected band size for Alpha B Crystallin/CRYAB is at 20KD.
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