Immunohistochemical analysis of human brain cortex tissue stained with alphaB-Crystallin pAb at 10 microg/mL.
Immunohistochemical analysis of human brain cortex tissue stained with alphaB-Crystallin pAb at 10 microg/mL.
alpha B Crystallin antibody
GTX13497
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Hamster, Human, Mouse, Porcine, Rabbit, Rat
TargetCRYAB
Overview
- SupplierGeneTex
- Product Namealpha B Crystallin antibody
- Delivery Days Customer9
- Antibody SpecificityThis antibody does not cross-react with beta Crystallin nor alpha A Crystallin.
- Application Supplier NoteRecommended Starting Dilutions:ICC: Use at a dilution of 1/200. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000 - 1/2000. 1/2000 dilution is sufficient to detect 50ng of alpha B Crystallin. Detects a band of approximately 22 kDa (predicted MW 20 kDa). Optimal dilutions/concentrations should be determined by the end user.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1410
- Target nameCRYAB
- Target descriptioncrystallin alpha B
- Target synonymsalpha-crystallin B chain; CMD1II; CRYA2; CTPP2; CTRCT16; epididymis secretory protein Li 101; heat shock protein beta-5; heat-shock 20 kD like-protein; HEL-S-101; HSPB5; MFM2; renal carcinoma antigen NY-REN-27; rosenthal fiber component
- HostRabbit
- IsotypeIgG
- Protein IDP02511
- Protein NameAlpha-crystallin B chain
- Scientific DescriptionMammalian lens crystallins are divided into alpha, beta, and gamma families. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha crystallins can be induced by heat shock and are members of the small heat shock protein (HSP20) family. They act as molecular chaperones although they do not renature proteins and release them in the fashion of a true chaperone; instead they hold them in large soluble aggregates. Post-translational modifications decrease the ability to chaperone. These heterogeneous aggregates consist of 30-40 subunits; the alpha-A and alpha-B subunits have a 3:1 ratio, respectively. Two additional functions of alpha crystallins are an autokinase activity and participation in the intracellular architecture. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alpha-A and alpha-B gene products are differentially expressed; alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. Elevated expression of alpha-B crystallin occurs in many neurological diseases; a missense mutation cosegregated in a family with a desmin-related myopathy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
- ReactivityBovine, Hamster, Human, Mouse, Porcine, Rabbit, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203