Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using alpha Glucosidase II(GTX102237) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
![Wild-type (WT) and alpha Glucosidase II knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Glucosidase II antibody [N1N2], N-term (GTX102237) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102237/GTX102237_39995_20181102_WB_KO_watermark_w_23060100_869.webp "Wild-type (WT) and alpha Glucosidase II knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with alpha Glucosidase II antibody [N1N2], N-term (GTX102237) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![alpha Glucosidase II antibody [N1N2], N-term detects alpha Glucosidase II protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. alpha Glucosidase II antibody [N1N2], N-term (GTX102237) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX102237/GTX102237_39995_20151223_IHC-P_w_23060100_168.webp "alpha Glucosidase II antibody [N1N2], N-term detects alpha Glucosidase II protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. alpha Glucosidase II antibody [N1N2], N-term (GTX102237) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using alpha Glucosidase II(GTX102237) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
alpha Glucosidase II antibody [N1N2], N-term
GTX102237
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetGANAB
Overview
- SupplierGeneTex
- Product Namealpha Glucosidase II antibody [N1N2], N-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID23193
- Target nameGANAB
- Target descriptionglucosidase II alpha subunit
- Target synonymsG2AN, GIIA, GIIalpha, GLUII, PKD3, neutral alpha-glucosidase AB, alpha-glucosidase 2
- HostRabbit
- IsotypeIgG
- Protein IDQ14697
- Protein NameNeutral alpha-glucosidase AB
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Timms RT, Menzies SA, Tchasovnikarova IA, et al. Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens. Nat Commun. 2016,7:11786. doi: 10.1038/ncomms11786Read this paper
Datasheet
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