More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/GAA Polyclonal Antibody
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Alpha Glucosidase Polyclonal Antibody (bs-13254R) at 1:200 overnight at 4°C, followed by a conjugated secondary.
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Alpha Glucosidase Polyclonal Antibody (bs-13254R) at 1:200 overnight at 4°C, followed by a conjugated secondary.
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Alpha Glucosidase Polyclonal Antibody (bs-13254R) at 1:200 overnight at 4°C, followed by a conjugated secondary.

GAA Polyclonal Antibody

BS-13254R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGAA
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Bioss
  • Product Name
    GAA Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID2548
  • Target name
    GAA
  • Target description
    alpha glucosidase
  • Target synonyms
    IOPD, LOPD, LYAG, lysosomal alpha-glucosidase, acid maltase, aglucosidase alfa, glucosidase alpha, acid
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP10253
  • Protein Name
    Lysosomal alpha-glucosidase
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Rauniyar N, Subramanian K, Lavallée-Adam M, et al. Quantitative Proteomics of Human Fibroblasts with I1061T Mutation in Niemann-Pick C1 (NPC1) Protein Provides Insights into the Disease Pathogenesis. Mol Cell Proteomics. 2015,14(7):1734-49. doi: 10.1074/mcp.M114.045609
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Immunohistochemistry of paraffin-embedded human prostate cancer using CSB-PA009125LA01HU at dilution of 1:100
Product group Antibodies
Cusabio
GAA AntibodyCSB-PA009125LA01HU
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetGAA
  • SizePrice
Product group Antibodies
MedChem Express
GAA Antibody (YA2872, Rabbit)HY-P83127
TargetGAA
  • SizePrice
Product group Antibodies
Biosynth
GAA antibody70R-17387
  • SizePrice
Product group Antibodies
RayBiotech
Anti-GAA Antibody144-07674
ApplicationsWestern Blot
TargetGAA
  • SizePrice
Anti-GAA Antibody
Product group Antibodies
Antibodies.com
Anti-GAA AntibodyA15834
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • SizePrice
Figure 1. Western blot analysis of GAA using anti-GAA antibody (A01548). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human PC-3 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GAA antigen affinity purified polyclonal antibody (Catalog # A01548) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GAA at approximately 110,95,76,70KD. The expected band size for GAA is at 110,95,76,70KD.
Product group Antibodies
Boster Bio
Anti-GAA Antibody Picoband(r)A01548-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetGAA
  • SizePrice
Product group Antibodies
References
GeneTex
LYAG antibody [C2C3], C-termGTX109821
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetGAA
  • SizePrice
Product group Antibodies
Assay Genie
GAA AntibodyCAB7674
TargetGAA
  • SizePrice
Product group Antibodies
Biomatik
Gaa Polyclonal AntibodyCAC07609
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetGAA
  • SizePrice
Product group Antibodies
Nordic-MUbio
Mouse anti alpha-GlucosidaseMUB0707P
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetGAA
  • SizePrice