Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Alpha Glucosidase Polyclonal Antibody (bs-13254R) at 1:200 overnight at 4°C, followed by a conjugated secondary.
Paraformaldehyde-fixed, paraffin embedded rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Alpha Glucosidase Polyclonal Antibody (bs-13254R) at 1:200 overnight at 4°C, followed by a conjugated secondary.
GAA Polyclonal Antibody
BS-13254R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetGAA
Overview
- SupplierBioss Antibodies
- Product NameAlpha Glucosidase Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID2548
- Target nameGAA
- Target descriptionalpha glucosidase
- Target synonymsacid maltase; aglucosidase alfa; glucosidase alpha, acid; LYAG; lysosomal alpha-glucosidase
- HostRabbit
- IsotypeIgG
- Protein IDP10253
- Protein NameLysosomal alpha-glucosidase
- Storage Instruction-20°C
- UNSPSC12352203
References
- Quantitative Proteomics of Human Fibroblasts with I1061T Mutation in Niemann-Pick C1 (NPC1) Protein Provides Insights into the Disease Pathogenesis. Rauniyar N et al., 2015 Jul, Mol Cell ProteomicsRead more
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