Wild-type (WT) and LYAG knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109821/GTX109821_43705_20190927_WB_22102723_528.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![LYAG antibody [C2C3], C-term detects LYAG protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. LYAG stained by LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX109821/GTX109821_43586_20190628_IHC-P_w_23060500_334.webp "LYAG antibody [C2C3], C-term detects LYAG protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. LYAG stained by LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![LYAG antibody [C2C3], C-term detects LYAG protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. LYAG stained by LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX109821/GTX109821_43705_20200221_IHC-P_w_23060500_121.webp "LYAG antibody [C2C3], C-term detects LYAG protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. LYAG stained by LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Wild-type (WT) and LYAG knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LYAG antibody [C2C3], C-term (GTX109821) diluted at 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
LYAG antibody [C2C3], C-term
GTX109821
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetGAA
Overview
- SupplierGeneTex
- Product NameLYAG antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.76 mg/ml
- ConjugateUnconjugated
- Gene ID2548
- Target nameGAA
- Target descriptionalpha glucosidase
- Target synonymsIOPD, LOPD, LYAG, lysosomal alpha-glucosidase, acid maltase, aglucosidase alfa, glucosidase alpha, acid
- HostRabbit
- IsotypeIgG
- Protein IDP10253
- Protein NameLysosomal alpha-glucosidase
- Scientific DescriptionThis gene encodes acid alpha-glucosidase, which is essential for the degradation of glycogen to glucose in lysosomes. Different forms of acid alpha-glucosidase are obtained by proteolytic processing. Defects in this gene are the cause of glycogen storage disease II, also known as Pompes disease, which is an autosomal recessive disorder with a broad clinical spectrum. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Napolitano F, Bruno G, Terracciano C, et al. Rare Variants in Autophagy and Non-Autophagy Genes in Late-Onset Pompe Disease: Suggestions of Their Disease-Modifying Role in Two Italian Families. Int J Mol Sci. 2021,22(7). doi: 10.3390/ijms22073625Read this paper
- Basile I, Da Silva A, El Cheikh K, et al. Efficient therapy for refractory Pompe disease by mannose 6-phosphate analogue grafting on acid α-glucosidase. J Control Release. 2018,269:15-23. doi: 10.1016/j.jconrel.2017.10.043Read this paper
Datasheet
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