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ProductsBack/alpha Internexin Recombinant Antibody
Lane 1: Mouse Bran; Lane 2: A549 Cells; Probed with alpha Internexin (1C7) Monoclonal Antibody (bsm-54173R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Mouse Bran; Lane 2: A549 Cells; Probed with alpha Internexin (1C7) Monoclonal Antibody (bsm-54173R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Lane 1: Mouse Bran; Lane 2: A549 Cells; Probed with alpha Internexin (1C7) Monoclonal Antibody (bsm-54173R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.

alpha Internexin Recombinant Antibody

BSM-54173R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetINA
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    alpha Internexin (1C7) Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), IHC-P(1:200-400), IHC-F(1:100-500), IF(), Flow-Cyt(1ug/Test), ICC/IF(1:50-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID9118
  • Target name
    INA
  • Target description
    internexin neuronal intermediate filament protein alpha
  • Target synonyms
    66 kDa neurofilament protein; alpha-internexin; alpha-Inx; NEF5; neurofilament 5 (66kD); neurofilament-66, tax-binding protein; NF66; NF-66; TXBP-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16352
  • Protein Name
    Alpha-internexin
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

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Figure 1. Western blot analysis of Alpha Internexin/INA using anti-Alpha Internexin/INA antibody (A03756-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Internexin/INA antigen affinity purified polyclonal antibody (Catalog # A03756-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Internexin/INA at approximately 66 kDa. The expected band size for Alpha Internexin/INA is at 66 kDa.
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