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alpha Internexin antibody detects alpha Internexin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted by 1:1000.
alpha Internexin antibody detects alpha Internexin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted by 1:1000.
alpha Internexin antibody detects alpha Internexin protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Internexin antibody (GTX130053) diluted by 1:1000.

alpha Internexin antibody

GTX130053
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetINA
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Overview

  • Supplier
    GeneTex
  • Product Name
    alpha Internexin antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IP: 1:100-1:800. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.66 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9118
  • Target name
    INA
  • Target description
    internexin neuronal intermediate filament protein alpha
  • Target synonyms
    NEF5, NF-66, NF66, TXBP-1, alpha-internexin, 66 kDa neurofilament protein, alpha-Inx, neurofilament 5 (66kD), neurofilament-66, tax-binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16352
  • Protein Name
    Alpha-internexin
  • Scientific Description
    Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Alpha Internexin/INA using anti-Alpha Internexin/INA antibody (A03756-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha Internexin/INA antigen affinity purified polyclonal antibody (Catalog # A03756-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha Internexin/INA at approximately 66 kDa. The expected band size for Alpha Internexin/INA is at 66 kDa.
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