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ALPL / Alkaline Phosphatase Antibody

LS-C761286
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetALPL
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ALPL / Alkaline Phosphatase Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:100), WB (1:500 - 1:1000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID249
  • Target name
    ALPL
  • Target description
    alkaline phosphatase, biomineralization associated
  • Target synonyms
    AP-TNAP, APTNAP, HOPS, HPPA, HPPC, HPPI, HPPO, TNALP, TNAP, TNS-ALP, TNSALP, alkaline phosphatase, tissue-nonspecific isozyme, alkaline phosphatase liver/bone/kidney isozyme, alkaline phosphatase, liver/bone/kidney, liver/bone/kidney-type alkaline phosphatase, phosphoamidase, phosphocreatine phosphatase, tissue non-specific alkaline phosphatase, tissue-nonspecific ALP
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Alkaline phosphatase/ALPL using anti-Alkaline phosphatase/ALPL antibody (A01008-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alkaline phosphatase/ALPL antigen affinity purified polyclonal antibody (Catalog # A01008-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alkaline phosphatase/ALPL at approximately 80KD. The expected band size for Alkaline phosphatase/ALPL is at 57KD.
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