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Western blot All lanes: Alkaline phosphatase, placental type antibody at 8ug/ml Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa
Western blot All lanes: Alkaline phosphatase, placental type antibody at 8ug/ml Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa
Western blot All lanes: Alkaline phosphatase, placental type antibody at 8ug/ml Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 kDa

ALPP Antibody

CSB-PA001632ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetALPP
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Overview

  • Supplier
    Cusabio
  • Product Name
    ALPP Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID250
  • Target name
    ALPP
  • Target description
    alkaline phosphatase, placental
  • Target synonyms
    alkaline phosphatase Regan isozyme; alkaline phosphatase, placental type; alkaline phosphomonoesterase; ALP; ALPI; glycerophosphatase; IAP; Intestinal alkaline phosphatase; Intestinal-type alkaline phosphatase; PALP; placental alkaline phosphatase 1; PLAP; PLAP-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05187
  • Protein Name
    Alkaline phosphatase, placental type
  • Scientific Description
    Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ALPP using anti-ALPP antibody (A01718). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALPP antigen affinity purified polyclonal antibody (Catalog # A01718) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALPP at approximately 70 kDa. The expected band size for ALPP is at 58 kDa.
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