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ALY antibody

70R-13574
Biosynth
Product group Antibodies
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Overview

  • Supplier
    Biosynth
  • Product Name
    ALY antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ALYREF using anti-ALYREF antibody (A03580-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat liver tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: rat NRK whole cell lysates, Lane 12: mouse brain tissue lysates, Lane 13: mouse liver tissue lysates, Lane 14: mouse Neuro-2a whole cell lysates, Lane 15: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALYREF antigen affinity purified polyclonal antibody (Catalog # A03580-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALYREF at approximately 35 kDa. The expected band size for ALYREF is at 27 kDa.
Product group Antibodies
Boster Bio
Anti-ALYREF Antibody Picoband(r)A03580-3-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetALYREF
  • SizePrice
Product group Antibodies
RayBiotech
Anti-ALYREF Antibody144-06010
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetALYREF
  • SizePrice
Product group Antibodies
Abbexa
ALYREF AntibodyABX333914
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
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Western Blot analysis of LOVO cells using ALY Polyclonal Antibody
Product group Antibodies
Cusabio
ALYREF AntibodyCSB-PA006422
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetALYREF
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Product group Antibodies
Assay Genie
THOC4/ALYREF Monoclonal AntibodyCAB4298
ReactivityHuman
TargetALYREF
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Product group Antibodies
Bioss
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ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetALYREF
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Product group Antibodies
LifeSpan BioSciences
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ReactivityHuman
TargetALYREF
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Product group Antibodies
MedChem Express
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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles.
Product group Antibodies
Atlas Antibodies
Anti-ALYREF AntibodyHPA061282
ApplicationsWestern Blot, ImmunoCytoChemistry
ReactivityHuman
TargetALYREF
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ALY antibody detects ALY protein at nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. ALY antibody (GTX113917) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Product group Antibodies
References
GeneTex
ALY antibodyGTX113917
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetALYREF
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