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ProductsBack/ALYREF Antibody
Western Blot analysis of LOVO cells using ALY Polyclonal Antibody
Western Blot analysis of LOVO cells using ALY Polyclonal Antibody
Western Blot analysis of LOVO cells using ALY Polyclonal Antibody

ALYREF Antibody

CSB-PA006422
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetALYREF
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Overview

  • Supplier
    Cusabio
  • Product Name
    ALYREF Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10189
  • Target name
    ALYREF
  • Target description
    Aly/REF export factor
  • Target synonyms
    ALY, ALY/REF, BEF, REF, THOC4, THO complex subunit 4, THO complex 4, ally of AML-1 and LEF-1, bZIP enhancing factor, bZIP-enhancing factor BEF, tho4, transcriptional coactivator Aly/REF
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86V81
  • Protein Name
    THO complex subunit 4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ALYREF using anti-ALYREF antibody (A03580-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human A431 whole cell lysates, Lane 7: human A549 whole cell lysates, Lane 8: rat brain tissue lysates, Lane 9: rat liver tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: rat NRK whole cell lysates, Lane 12: mouse brain tissue lysates, Lane 13: mouse liver tissue lysates, Lane 14: mouse Neuro-2a whole cell lysates, Lane 15: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALYREF antigen affinity purified polyclonal antibody (Catalog # A03580-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ALYREF at approximately 35 kDa. The expected band size for ALYREF is at 27 kDa.
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