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AMACR antibody

10R-1851
Biosynth
Product group Antibodies
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ISO certified
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Overview

  • Supplier
    Biosynth
  • Product Name
    AMACR antibody
  • Delivery Days Customer
    11
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Lane 1: Mouse Liver tissue lysates; Lane 2: Mouse Kidney tissue lysates; Lane 3: Rat Liver tissue lysates; Lane 4: Rat Kidney tissue lysates probed with AMACR Polyclonal Antibody, Unconjugated (bs-0840R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of AMHR2 using anti-AMHR2 antibody (PB9983). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat kidney tissue lysates. Lane 2: rat liver tissue lysates. Lane 3: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMHR2 antigen affinity purified polyclonal antibody (Catalog # PB9983) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002)?with Tanon 5200 system. A specific band was detected for AMHR2 at approximately 42 kDa. The expected band size for AMHR2 is at 42 kDa.
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