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AMBP Antibody (YA3369, Rabbit)

HY-P83624
MedChem Express
TargetAMBP
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    AMBP Antibody (YA3369, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID259
  • Target name
    AMBP
  • Target description
    alpha-1-microglobulin/bikunin precursor
  • Target synonyms
    A1M, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI, protein AMBP, bikunin, complex-forming glycoprotein heterogeneous in charge, growth-inhibiting protein 19, inter-alpha-trypsin inhibitor light chain, protein HC, trypstatin, uristatin, uronic-acid-rich protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    AMBP Antibody (YA3369) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3369), targeting AMBP, with a predicted molecular weight of 39 kDa (observed band size: 39 kDa). AMBP Antibody (YA3369) can be used for WB, IHC-P experiment in human background.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Bikunin/AMBP using anti-Bikunin/AMBP antibody (A02419-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bikunin/AMBP antigen affinity purified polyclonal antibody (Catalog # A02419-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bikunin/AMBP at approximately 34KD. The expected band size for Bikunin/AMBP is at 39KD.
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