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Western Blot analysis of Human testis tissue using AMBP Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Human testis tissue using AMBP Polyclonal Antibody at dilution of 1:500
Western Blot analysis of Human testis tissue using AMBP Polyclonal Antibody at dilution of 1:500

AMBP Polyclonal Antibody

E-AB-10779
Elabscience
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetAMBP
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Overview

  • Supplier
    Elabscience
  • Product Name
    AMBP Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID259
  • Target name
    AMBP
  • Target description
    alpha-1-microglobulin/bikunin precursor
  • Target synonyms
    A1M, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI, protein AMBP, bikunin, complex-forming glycoprotein heterogeneous in charge, growth-inhibiting protein 19, inter-alpha-trypsin inhibitor light chain, protein HC, trypstatin, uristatin, uronic-acid-rich protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP02760
  • Protein Name
    Protein AMBP
  • Scientific Description
    This gene encodes a complex glycoprotein secreted in plasma. The precursor is proteolytically processed into distinct functioning proteins: alpha-1-microglobulin, which belongs to the superfamily of lipocalin transport proteins and may play a role in the regulation of inflammatory processes, and bikunin, which is a urinary trypsin inhibitor belonging to the superfamily of Kunitz-type protease inhibitors and plays an important role in many physiological and pathological processes. This gene is located on chromosome 9 in a cluster of lipocalin genes.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

MSDS

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Figure 1. Western blot analysis of Bikunin/AMBP using anti-Bikunin/AMBP antibody (A02419-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bikunin/AMBP antigen affinity purified polyclonal antibody (Catalog # A02419-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bikunin/AMBP at approximately 34KD. The expected band size for Bikunin/AMBP is at 39KD.
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