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Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate All lanes: AMFR antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73 kDa Observed band size: 73 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate All lanes: AMFR antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73 kDa Observed band size: 73 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate, Hela whole cell lysate All lanes: AMFR antibody at 4microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 73 kDa Observed band size: 73 kDa

AMFR Antibody

CSB-PA333164LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetAMFR
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Overview

  • Supplier
    Cusabio
  • Product Name
    AMFR Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID267
  • Target name
    AMFR
  • Target description
    autocrine motility factor receptor
  • Target synonyms
    E3 ubiquitin-protein ligase AMFR; GP78; RING finger protein 45; RING-type E3 ubiquitin transferase AMFR; RNF45
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UKV5
  • Protein Name
    E3 ubiquitin-protein ligase AMFR
  • Scientific Description
    E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97-AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97-AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also regulates ERAD through the ubiquitination of UBL4A a component of the BAG6/BAT3 complex. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis as a receptor for the GPI/autocrine motility factor.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AMFR using anti-AMFR antibody (PB10039). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Daudi whole cell lysates, Lane 4: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMFR antigen affinity purified polyclonal antibody (Catalog # PB10039) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMFR at approximately 73 kDa. The expected band size for AMFR is at 73 kDa.
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