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WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX55509 AMOT antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX55509 AMOT antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX55509 AMOT antibody. Dilution : 1:1000 Loading : 25microg per lane

AMOT antibody

GTX55509
GeneTex
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetAMOT
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Overview

  • Supplier
    GeneTex
  • Product Name
    AMOT antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID154796
  • Target name
    AMOT
  • Target description
    angiomotin
  • Target synonyms
    angiomotin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ4VCS5
  • Protein Name
    Angiomotin
  • Scientific Description
    This gene belongs to the motin family of angiostatin binding proteins characterized by conserved coiled-coil domains and C-terminal PDZ binding motifs. The encoded protein is expressed predominantly in endothelial cells of capillaries as well as larger vessels of the placenta where it may mediate the inhibitory effect of angiostatin on tube formation and the migration of endothelial cells toward growth factors during the formation of new blood vessels. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Angiomotin/AMOT using anti-Angiomotin/AMOT antibody (A04170-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiomotin/AMOT antigen affinity purified polyclonal antibody (Catalog # A04170-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiomotin/AMOT at approximately 140 kDa. The expected band size for Angiomotin/AMOT is at 118 kDa.
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