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Western Blot Positive WB detected in: 293T whole cell lysate, U251 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate All lanes: AMOTL2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86, 92 kDa Observed band size: 86 kDa
Western Blot Positive WB detected in: 293T whole cell lysate, U251 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate All lanes: AMOTL2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86, 92 kDa Observed band size: 86 kDa
Western Blot Positive WB detected in: 293T whole cell lysate, U251 whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, MCF-7 whole cell lysate, PC3 whole cell lysate All lanes: AMOTL2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 86, 92 kDa Observed band size: 86 kDa

AMOTL2 Antibody

CSB-PA809099NA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetAMOTL2
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Overview

  • Supplier
    Cusabio
  • Product Name
    AMOTL2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID51421
  • Target name
    AMOTL2
  • Target description
    angiomotin like 2
  • Target synonyms
    angiomotin-like protein 2; LCCP; Leman coiled-coil protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2J4
  • Protein Name
    Angiomotin-like protein 2
  • Scientific Description
    Regulates the translocation of phosphorylated SRC to peripheral cell-matrix adhesion sites. Required for proper architecture of actin filaments. Inhibits the Wnt/beta-catenin signaling pathway, probably by recruiting CTNNB1 to recycling endosomes and hence preventing its translocation to the nucleus. Participates in angiogenesis. May play a role in the polarity, proliferation and migration of endothelial cells. Selectively promotes FGF-induced MAPK activation through SRC.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AMOTL2 using anti-AMOTL2 antibody (A06852). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMOTL2 antigen affinity purified polyclonal antibody (Catalog # A06852) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMOTL2 at approximately 95KD. The expected band size for AMOTL2 is at 86KD.
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