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Anti-AMOTL2 Antibody

144-64614
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetAMOTL2
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-AMOTL2 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Gene ID51421
  • Target name
    AMOTL2
  • Target description
    angiomotin like 2
  • Target synonyms
    angiomotin-like protein 2; LCCP; Leman coiled-coil protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2J4
  • Protein Name
    Angiomotin-like protein 2
  • Scientific Description
    AMOTL2 pAb
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AMOTL2 using anti-AMOTL2 antibody (A06852). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMOTL2 antigen affinity purified polyclonal antibody (Catalog # A06852) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMOTL2 at approximately 95KD. The expected band size for AMOTL2 is at 86KD.
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