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Western Blot Positive WB detected in: MCF-7 whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate All lanes: AMPD1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 91, 90 kDa Observed band size: 91 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate All lanes: AMPD1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 91, 90 kDa Observed band size: 91 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, PC-3 whole cell lysate, A549 whole cell lysate All lanes: AMPD1 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 91, 90 kDa Observed band size: 91 kDa

AMPD1 Antibody

CSB-PA001680LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetAMPD1
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Overview

  • Supplier
    Cusabio
  • Product Name
    AMPD1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID270
  • Target name
    AMPD1
  • Target description
    adenosine monophosphate deaminase 1
  • Target synonyms
    adenosine monophosphate deaminase 1 (isoform M); adenosine monophosphate deaminase-1 (muscle); AMP deaminase 1; AMPD; MAD; MADA; MMDD; myoadenylate deaminase; skeletal muscle AMPD
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP23109
  • Protein Name
    AMP deaminase 1
  • Scientific Description
    AMP deaminase plays a critical role in energy metabolism.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of AMPD1 using anti-AMPD1 antibody (A04209-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPD1 antigen affinity purified polyclonal antibody (Catalog # A04209-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPD1 at approximately 86 kDa. The expected band size for AMPD1 is at 86 kDa.
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