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Goat anti-AMPD1

EB08258
Everest Biotech
ApplicationsELISA
Product group Antibodies
TargetAMPD1
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-AMPD1 Antibody
  • Delivery Days Customer
    5
  • Applications
    ELISA
  • Applications Supplier
    Pep-ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID270
  • Target name
    AMPD1
  • Target description
    adenosine monophosphate deaminase 1
  • Target synonyms
    adenosine monophosphate deaminase 1 (isoform M); adenosine monophosphate deaminase-1 (muscle); AMP deaminase 1; AMPD; MAD; MADA; MMDD; myoadenylate deaminase; skeletal muscle AMPD
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000027.1. Purification: Antigen affinity purified. Names and symbols: AMPD1; adenosine monophosphate deaminase 1 (isoform M); MAD; MADA; Adenosine monophosphate deaminase-1 (muscle); OTTHUMP00000059283
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Cow, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of AMPD1 using anti-AMPD1 antibody (A04209-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat heart tissue lysates, Lane 4: rat skeletal muscle tissue lysates, Lane 5: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMPD1 antigen affinity purified polyclonal antibody (Catalog # A04209-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AMPD1 at approximately 86 kDa. The expected band size for AMPD1 is at 86 kDa.
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