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Western Blot analysis of Mouse lung tissue using Amphiphysin I Polyclonal Antibody at dilution of 1:400
Western Blot analysis of Mouse lung tissue using Amphiphysin I Polyclonal Antibody at dilution of 1:400
Western Blot analysis of Mouse lung tissue using Amphiphysin I Polyclonal Antibody at dilution of 1:400

Amphiphysin I Polyclonal Antibody

E-AB-10128
Elabscience
ApplicationsWestern Blot
Product group Antibodies
TargetAMPH
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Overview

  • Supplier
    Elabscience
  • Product Name
    Amphiphysin I Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID273
  • Target name
    AMPH
  • Target description
    amphiphysin
  • Target synonyms
    AMPH1, amphiphysin, amphiphysin (Stiff-Mann syndrome with breast cancer 128kD autoantigen), amphiphysin I
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49418
  • Protein Name
    Amphiphysin
  • Scientific Description
    This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Amphiphysin/AMPH using anti-Amphiphysin/AMPH antibody (A02364-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiphysin/AMPH antigen affinity purified polyclonal antibody (Catalog # A02364-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiphysin/AMPH at approximately 115-125 kDa. The expected band size for Amphiphysin/AMPH is at 76 kDa.
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