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Western Blot analysis of Mouse lung tissue using Amphiphysin I Polyclonal Antibody at dilution of 1:400
Western Blot analysis of Mouse lung tissue using Amphiphysin I Polyclonal Antibody at dilution of 1:400
Western Blot analysis of Mouse lung tissue using Amphiphysin I Polyclonal Antibody at dilution of 1:400

AMPH Polyclonal Antibody

E-AB-10128
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    AMPH Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Concentration
    0.3mg/ml
  • Scientific Description
    This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Amphiphysin/AMPH using anti-Amphiphysin/AMPH antibody (A02364-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Amphiphysin/AMPH antigen affinity purified polyclonal antibody (Catalog # A02364-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Amphiphysin/AMPH at approximately 115-125 kDa. The expected band size for Amphiphysin/AMPH is at 76 kDa.
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Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Amphiphysin Polyclonal Antibody, Unconjugated (bs-2409R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
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