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Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using APC5 Polyclonal Antibody at dilution 1:30
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using APC5 Polyclonal Antibody at dilution 1:30
Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using APC5 Polyclonal Antibody at dilution 1:30

ANAPC5 Polyclonal Antibody

E-AB-10810
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    ANAPC5 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.3mg/ml
  • Scientific Description
    This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Apc5/ANAPC5 using anti-Apc5/ANAPC5 antibody (A08280-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apc5/ANAPC5 antigen affinity purified polyclonal antibody (Catalog # A08280-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apc5/ANAPC5 at approximately 85KD. The expected band size for Apc5/ANAPC5 is at 85KD.
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