More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/ANAPC5 Polyclonal Antibody

ANAPC5 Polyclonal Antibody

RD84295A
Reddot Biotech
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetANAPC5
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Reddot Biotech
  • Product Name
    ANAPC5 Polyclonal Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51433
  • Target name
    ANAPC5
  • Target description
    anaphase promoting complex subunit 5
  • Target synonyms
    anaphase-promoting complex subunit 5; APC5; cyclosome subunit 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C), a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b), compared to isoform a.
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Assay Genie
ANAPC5 AntibodyCAB7109
TargetANAPC5
  • SizePrice
Western Blot analysis of COLO205 cells using APC5 Polyclonal Antibody
Product group Antibodies
Cusabio
ANAPC5 AntibodyCSB-PA000899
ApplicationsWestern Blot, ELISA
TargetANAPC5
  • SizePrice
Anti-ANAPC5 Antibody
Product group Antibodies
Antibodies.com
Anti-ANAPC5 AntibodyA100614
ApplicationsWestern Blot, ELISA
  • SizePrice
Figure 1. Western blot analysis of Apc5/ANAPC5 using anti-Apc5/ANAPC5 antibody (A08280-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human HEK293 whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Jurkat whole cell lysates, Lane 5: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Apc5/ANAPC5 antigen affinity purified polyclonal antibody (Catalog # A08280-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Apc5/ANAPC5 at approximately 85KD. The expected band size for Apc5/ANAPC5 is at 85KD.
Product group Antibodies
Boster Bio
Anti-Apc5/ANAPC5 Antibody Picoband(r)A08280-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
TargetANAPC5
  • SizePrice
WB analysis of human kidney tissue lysate using GTX17060 ANAPC5 antibody. Working concentration : (A) 1 and (B) 2 microg/ml
Product group Antibodies
GeneTex
APC5 antibodyGTX17060
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetANAPC5
  • SizePrice
Product group Antibodies
RayBiotech
Anti-ANAPC5 Antibody144-07109
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetANAPC5
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-APC5 Antibody, Affinity PurifiedA301-026A
ApplicationsImmunoPrecipitation, Western Blot
TargetANAPC5
  • SizePrice
Product group Antibodies
Bioss Antibodies
APC5 Polyclonal AntibodyBS-7778R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetANAPC5
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ANAPC5 / APC5 AntibodyLS-C401258
ApplicationsELISA, ImmunoHistoChemistry
TargetANAPC5
  • SizePrice
Immunohistochemical staining of human fallopian tube shows nuclear membrane positivity in glandular cells.
Product group Antibodies
Atlas Antibodies
Anti-ANAPC5 AntibodyHPA039457
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetANAPC5
  • SizePrice