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ProductsBack/Androgen Receptor (AR) Mouse Monoclonal Antibody [Clone ID: 1A9D12]
Western blot analysis using AR Cat.-No AM06279SU-N against truncated Trx-AR recombinant protein (Lane 1).
Western blot analysis using AR Cat.-No AM06279SU-N against truncated Trx-AR recombinant protein (Lane 1).
Western blot analysis using AR Cat.-No AM06279SU-N against truncated Trx-AR recombinant protein (Lane 1).

Androgen Receptor (AR) Mouse Monoclonal Antibody [Clone ID: 1A9D12]

AM06279SU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetAR
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    Androgen Receptor (AR) Mouse Monoclonal Antibody [Clone ID: 1A9D12]
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1A9D12
  • Gene ID367
  • Target name
    AR
  • Target description
    androgen receptor
  • Target synonyms
    AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM, androgen receptor, dihydrotestosterone receptor, nuclear receptor subfamily 3 group C member 4
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP10275
  • Protein Name
    Androgen receptor
  • Scientific Description
    Androgen Receptor (AR) mouse monoclonal antibody, clone 1A9D12, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Androgen Receptor/AR using anti-Androgen Receptor/AR antibody (A00542). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human U20S whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human PC-3 whole cell lysates, Lane 5: human 22RV1 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Androgen Receptor/AR antigen affinity purified polyclonal antibody (Catalog # A00542) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Androgen Receptor/AR at approximately 120KD. The expected band size for Androgen Receptor/AR is at 120KD.
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