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Angiopoietin-like 4 antibody [N1C2]

GTX114198
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetANGPTL4
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Overview

  • Supplier
    GeneTex
  • Product Name
    Angiopoietin-like 4 antibody [N1C2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51129
  • Target name
    ANGPTL4
  • Target description
    angiopoietin like 4
  • Target synonyms
    angiopoietin-related protein 4; ARP4; fasting-induced adipose factor; FIAF; HARP; hepatic angiopoietin-related protein; hepatic fibrinogen/angiopoietin-related protein; HFARP; NL2; peroxisome proliferator-activated receptor (PPAR) gamma induced angiopoietin-related protein; PGAR; pp1158; PPARG angiopoietin related protein; TGQTL; UNQ171
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BY76
  • Protein Name
    Angiopoietin-related protein 4
  • Scientific Description
    This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. The encoded protein is a serum hormone directly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity and also acts as an apoptosis survival factor for vascular endothelial cells. The encoded protein may play a role in several cancers and it also has been shown to prevent the metastatic process by inhibiting vascular activity as well as tumor cell motility and invasiveness. Decreased expression of this protein has been associated with type 2 diabetes. Alternatively spliced transcript variants encoding different isoforms have been described. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Drug-selected population in melanoma A2058 cells as melanoma stem-like cells retained angiogenic features - the potential roles of heparan-sulfate binding ANGPTL4 protein. Shih CY et al., 2020 Nov 10, Aging (Albany NY)
    Read more
  • Hypoxia-induced angiopoietin-like protein 4 as a clinical biomarker and treatment target for human prostate cancer. Hata S et al., 2017 Jul, Oncol Rep
    Read more

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Figure 1. Western blot analysis of Angiopoietin-like 4/ANGPTL4 using anti-Angiopoietin-like 4/ANGPTL4 antibody (A01147-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin-like 4/ANGPTL4 antigen affinity purified polyclonal antibody (Catalog # A01147-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiopoietin-like 4/ANGPTL4 at approximately 45 kDa. The expected band size for Angiopoietin-like 4/ANGPTL4 is at 45 kDa.
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