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Anti-ANGPTL4 Antibody

Anti-ANGPTL4 Antibody

A96600
Antibodies.com
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-ANGPTL4 Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to ANGPTL4.
  • Reactivity
    Human, Mouse, Rat
  • UNSPSC
    12352203

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Western blot analysis using anti-ANGPTL4 (CCD) (human), mAb (Kairos4-153AD) (Prod. No. AG-20A-0046) at 1:500 dilution.1: Human ANGPTL4 (CCD) (FLAG®-tagged).2: Human ANGPTL4 (FLD) (FLAG®-tagged).3: Human ANGPTL4 (FLA
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Figure 1. Western blot analysis of Angiopoietin-like 4/ANGPTL4 using anti-Angiopoietin-like 4/ANGPTL4 antibody (A01147-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates. red to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin-like 4/ANGPTL4 antigen affinity purified polyclonal antibody (Catalog # A01147-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiopoietin-like 4/ANGPTL4 at approximately 45 kDa. The expected band size for Angiopoietin-like 4/ANGPTL4 is at 45 kDa.
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Anti-Angiopoietin-like 4/ANGPTL4 Antibody Picoband(r)A01147-2-CARRIER-FREE
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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Angiopoietin-like 4 antibody [N1C2] (GTX114198) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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