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ProductsBack/Angiotensin II type 1A receptor Polyclonal Antibody
Lane 1: Mouse liver lysates; Lane 2: Mouse lung lysate; Lane 3: mouse adrenal gland lysate probed with Angiotensin 2 type 1A receptor Polyclonal Antibody, Unconjugated (bs-2132R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Lane 1: Mouse liver lysates; Lane 2: Mouse lung lysate; Lane 3: mouse adrenal gland lysate probed with Angiotensin 2 type 1A receptor Polyclonal Antibody, Unconjugated (bs-2132R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C
Lane 1: Mouse liver lysates; Lane 2: Mouse lung lysate; Lane 3: mouse adrenal gland lysate probed with Angiotensin 2 type 1A receptor Polyclonal Antibody, Unconjugated (bs-2132R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Angiotensin II type 1A receptor Polyclonal Antibody

BS-2132R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetAGTR1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Angiotensin 2 type 1A receptor Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID185
  • Target name
    AGTR1
  • Target description
    angiotensin II receptor type 1
  • Target synonyms
    AG2S; AGTR1B; AT1; AT1AR; AT1B; AT1BR; AT1R; AT2R1; HAT1R; type-1 angiotensin II receptor; type-1B angiotensin II receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30556
  • Protein Name
    Type-1 angiotensin II receptor
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Angiotensin II subtype 1a receptor signaling in resident hepatic macrophages induces liver metastasis formation. Shimizu Y et al., 2017 Sep, Cancer Sci
    Read more

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Figure 1. Western blot analysis of AGTR1 using anti-AGTR1 antibody (PB9470). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: monkey COS-7 whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat liver tissue lysates, Lane 9: rat kidney tissue lysates, Lane 10: rat RH35 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse liver tissue lysates, Lane 13: mouse kidney tissue lysates, Lane 14: mouse HEPA1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGTR1 antigen affinity purified polyclonal antibody (Catalog # PB9470) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AGTR1 at approximately 50 kDa. The expected band size for AGTR1 is at 41 kDa.
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