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The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA337817(ANGPTL2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA337817(ANGPTL2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using CSB-PA337817(ANGPTL2 Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200)

ANGPTL2 Antibody

CSB-PA337817
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetANGPTL2
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Overview

  • Supplier
    Cusabio
  • Product Name
    ANGPTL2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23452
  • Target name
    ANGPTL2
  • Target description
    angiopoietin like 2
  • Target synonyms
    angiopoietin-like protein 2; angiopoietin-related protein 2; ARP2; HARP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UKU9
  • Protein Name
    Angiopoietin-related protein 2
  • Scientific Description
    Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ANGPTL2 using anti-ANGPTL2 antibody (A05747-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat stomach tissue lysate, lane 2: mouse ovary tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANGPTL2 antigen affinity purified polyclonal antibody (Catalog # A05747-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANGPTL2 at approximately 57KD. The expected band size for ANGPTL2 is at 57KD.
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