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Immunohistochemical staining of human stomach, upper shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach, upper shows strong membranous and cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach, upper shows strong membranous and cytoplasmic positivity in glandular cells.

Anti-ANGPTL2 Antibody

HPA040933
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetANGPTL2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ANGPTL2
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23452
  • Target name
    ANGPTL2
  • Target description
    angiopoietin like 2
  • Target synonyms
    angiopoietin-like protein 2; angiopoietin-related protein 2; ARP2; HARP
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UKU9
  • Protein Name
    Angiopoietin-related protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ANGPTL2 using anti-ANGPTL2 antibody (A05747-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: rat stomach tissue lysate, lane 2: mouse ovary tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANGPTL2 antigen affinity purified polyclonal antibody (Catalog # A05747-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANGPTL2 at approximately 57KD. The expected band size for ANGPTL2 is at 57KD.
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