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Western Blot analysis of Mouse lung tissue, NIH/3T3 and hepg2 cell using ANKRD1 Polyclonal Antibody at dilution of 1:400
Western Blot analysis of Mouse lung tissue, NIH/3T3 and hepg2 cell using ANKRD1 Polyclonal Antibody at dilution of 1:400
Western Blot analysis of Mouse lung tissue, NIH/3T3 and hepg2 cell using ANKRD1 Polyclonal Antibody at dilution of 1:400

ANKRD1 Polyclonal Antibody

E-AB-10801
Elabscience
ApplicationsWestern Blot
Product group Antibodies
TargetANKRD1
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Overview

  • Supplier
    Elabscience
  • Product Name
    ANKRD1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    Western Blot
  • Applications Supplier
    ELISA WB IHC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.3 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID27063
  • Target name
    ANKRD1
  • Target description
    ankyrin repeat domain 1
  • Target synonyms
    ALRP, C-193, CARP, CVARP, MCARP, bA320F15.2, ankyrin repeat domain-containing protein 1, ankyrin repeat domain 1 (cardiac muscle), cardiac ankyrin repeat protein, cytokine-inducible gene C-193 protein, cytokine-inducible nuclear protein, epididymis secretory sperm binding protein, liver ankyrin repeat domain 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ15327
  • Protein Name
    Ankyrin repeat domain-containing protein 1
  • Scientific Description
    The protein encoded by this gene is localized to the nucleus of endothelial cells and is induced by IL-1 and TNF-alpha stimulation. Studies in rat cardiomyocytes suggest that this gene functions as a transcription factor. Interactions between this protein and the sarcomeric proteins myopalladin and titin suggest that it may also be involved in the myofibrillar stretch-sensor system.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ANKRD1 using anti-ANKRD1 antibody (A04671-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat heart tissue lysates, Lane 2: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANKRD1 antigen affinity purified polyclonal antibody (Catalog # A04671-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ANKRD1 at approximately 36 kDa. The expected band size for ANKRD1 is at 36 kDa.
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