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The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA168510(ANLN Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA168510(ANLN Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)
The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA168510(ANLN Antibody) at dilution 1/20, on the right is treated with fusion protein. (Original magnification: x200)

ANLN Antibody

CSB-PA168510
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetANLN
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Overview

  • Supplier
    Cusabio
  • Product Name
    ANLN Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54443
  • Target name
    ANLN
  • Target description
    anillin actin binding protein
  • Target synonyms
    anillin; FSGS8; scra; Scraps
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NQW6
  • Protein Name
    Anillin
  • Scientific Description
    Anillin, also known as scraps homolog, is an evolutionarily conserved Actin- binding protein required for cytokinesis that was first identified in Drosophila melanogaster. Anillin is a ubiquitously expressed protein with highest expression levels in the central nervous system. It is predominantly found in the nucleus and it localizes to the cleavage furrow during cytokinesis, forming a ring with the help of Rac GTPase. During cytokinesis, Anillin interacts with CD2AP and functions to concentrate Rho A and maintain the localization of active Myosin. In Anillin knockout cells the cleavage furrow fails to complete ingression. Anillin expression levels fluctuate with the cell cycle, peaking in mitosis.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Formalin-fixed and paraffin embedded human placenta labeled with Anti-Anillin Polyclonal Antibody, Unconjugated (bs-7738R) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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Figure 1. Western blot analysis of Anillin/ANLN using anti-Anillin/ANLN antibody (A03997-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Anillin/ANLN antigen affinity purified polyclonal antibody (Catalog # A03997-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Anillin/ANLN at approximately 150-160 kDa. The expected band size for Anillin/ANLN is at 123 kDa.
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