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ICC/IF analysis of PFA-fixed HepG2 cells using GTX04983 Anillin antibody. Red : Primary antibody Green : Beta tubulin Blue : DAPI Permeabilization : 0.1% Triton X-100
ICC/IF analysis of PFA-fixed HepG2 cells using GTX04983 Anillin antibody. Red : Primary antibody Green : Beta tubulin Blue : DAPI Permeabilization : 0.1% Triton X-100
ICC/IF analysis of PFA-fixed HepG2 cells using GTX04983 Anillin antibody. Red : Primary antibody Green : Beta tubulin Blue : DAPI Permeabilization : 0.1% Triton X-100

Anillin antibody

GTX04983
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetANLN
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Overview

  • Supplier
    GeneTex
  • Product Name
    Anillin antibody
  • Delivery Days Customer
    7
  • Application Supplier Note
    WB: 1:500-1:2000. ICC/IF: 1:100-1:500. IHC-P: 1:50-1:200. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID54443
  • Target name
    ANLN
  • Target description
    anillin, actin binding protein
  • Target synonyms
    FSGS8, Scraps, scra, anillin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NQW6
  • Protein Name
    Anillin
  • Scientific Description
    This gene encodes an actin-binding protein that plays a role in cell growth and migration, and in cytokinesis. The encoded protein is thought to regulate actin cytoskeletal dynamics in podocytes, components of the glomerulus. Mutations in this gene are associated with focal segmental glomerulosclerosis 8. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Anillin/ANLN using anti-Anillin/ANLN antibody (A03997-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Anillin/ANLN antigen affinity purified polyclonal antibody (Catalog # A03997-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Anillin/ANLN at approximately 150-160 kDa. The expected band size for Anillin/ANLN is at 123 kDa.
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